Coding

Part:BBa_K4428004:Design

Designed by: Dhwani Teckchandani   Group: iGEM22_IIT-Delhi   (2022-10-13)


Surface Display of PbrR-MBD using BclB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 321
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 112


Design Notes

PbrR is downstream of BclB


Source

PbrR-MBD is derived from Cupriavidus metallidurans and BclB from Bacillus anthracis.

References